Week 6:
University of Iowa Department of Biochemistry

June 25, 2013

A Western Blot was run on the eluate from the crude filtration and several fractions from the gel chromatography; unfortunately it showed that while the Eluate from the crude filtration did indeed have FANCJ in it, none of the fractions did.  A second Western blot was run on the remaining fractions to determine if any of them contained FANCJ.  The two Western Blots together showed that none of the fractions contained FANCJ.  It was then determined that a SDS-Page gel should be run on the fractions most likely to contain FANCJ based on typical elution volumes for proteins of a similar size (in kDa).

Will Jones, one of the research assistants in the lab was very helpful in figuring out how to confirm the presence or absence of FANCJ in my samples.  It is great to be working in a lab where everyone is willing to help with a problem and find the solution.