Week 7:
University of Iowa Department of Biochemistry

July 3, 2013

The fractions most likely to contain FANCJ were run on an SDS-Page gel which showed a very faint signal in Fraction 7 for FANCJ.  Fraction 7 was analyzed on the Nanodrop spectrophotometer at 280 nm wavelength but there was no detectable concentration of FANCJ.  Because the concentration of FANCJ in the fraction was so low as to be unusable for the planned experiments, a new cell line of HEK293T was started from frozen stock.

This week was both stimulating and frustrating.  Frustrating because my preparation was not ready for experimentation and stimulating because Masa and I had to work on figuring out a way to increase the concentration of FANCJ produced by the transfection.  It’s always amazing to think about the differences between lab at the University of Iowa and at Cornell.  Labs at Cornell are great, but what happens is what happens and what you write up.  With more time to research, there is time to try procedures again, modify them and optimize their results.